Regulatory

Part:BBa_J100346

Designed by: Andrew DeRubertis   Group: Campbell M Lab   (2018-02-08)


Promoter for Reversal of Transcription of Pclone-Red in E.coli

This is a promoter that is effective in E. coli. Using Type IIs enzyme, BsaI, we removed the original promoter from Pclone Red plasmid. This original promoter started transcription in the direction of the GFP gene, and thus, when grown in E. coli, the cells turn green. After removing this promoter with BsaI, our promoter attaches to the plasmid using ligase, and reverses the transcription so that the plasmid will transcribe in the direction of RFP and the cells grown in the E. coli will turn red.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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